It is based on the measurement of unstable blue co lour produced by vitamin A soln in an anhydrous alcohol free chloroform and a sat a soln of antimony dichloride in anhydrous alcohol free CHcl3 maximum absorpn is observed around 620 nm. The blue co lour is very unstable and is necessary to determine within 8-10 sec. after addition of reagent. The reagent should be handled carefully as it is corrosive apparatus must be clean and dry as reagent is V sensitive to moisture.
Thoroughly purified vitamin A is a viscous yellow oil freely sol in methanol, ether, acetone and CHcl3 it is highly susceptible to atmospheric O2 . It is destroyed by UV light etherification of along. of vitamin A with palm tic or acetic acid produces some what stable comp. Hence vitamin A is prepared as oily solns. of acetate or palm tic.
1 iu = 0.344 mg of vitamin A acetate
= 0.3 ug of vitamin A.
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