Custom Search

Sunday, December 13, 2009

Estimate blood serum cholesterol Lieberman Buchan reaction

To estimate blood (or serum) cholesterol by Lieberman-Buchan reaction. Acetic anhydride reacts with cholesterol in CHCI3 soln to produce a characteristic blue green co lour. The exact nature of chromospheres is not known but the reaction probably includes etherification of hydroxyl group in the 3 – posn as well as other in the mol.
Cholesterol is abs-from the diet (about 0.3 mgm/day) as well as is syn. In the body (1.5 – 2 mg/day) free cholesterol is equally distributed b/w the cells and the plasma while the etherification form occurs only in plasma. The normal serum cholesterol lies with in the rang 100-250 mgm/100ml. The average serum cholesterol is 200 mgs/100ml at the age of 25 ml in men and rises slowly with age reading a peak figure at age of 40-50 and then declining women in generation show a lower cholesterol level than men until monoplane. When the value rises above that found in men of the same age.

Note: - It is essential to use absolutely dry glassware for this estimation.

Requirements: - Std cholesterol soln (1 mg/ml). Chloroform, acetic anhydride, conc. H2SO4, blood or serum.

Procedure: - Prepare series of test tube, as given Add all the soln from burette mix the soln in each tube and plug with cork and keep in dark for 15 mins measure the co lour developed at 680 nm against blank.
Isolation of blood (or serum) cholesterol. Add 0.2 ml of serum or bld. to 10 ml. mix of acetone alcohol mix(1:1) in a centrifuge tube keep the tube in boiling water bath with intermittent shaking till the solvent begin to boil. Remove the tube the W.B.shake it for 10 min and centrifuge decant the supernatant liquid into a dry t.t.and evap. To dryness on a W.B. cool and dissolve in 2 ml chloroform. Use 1 ml for co lour development. Cholesterol level in blood is det to follow the treatment of diseases like hypo hyperthyroidism diabetes, coronary artery diseases and arthero scleroses.

This website is a guide for for pharmacy students , pharmaceutical professional working in pharmaceutical regulatory affairs , pharmaceutical manufacturing , quality assurance , pharmaceutical formulation development , research and development.
This week we are publishing a series of articles on biochemistry, over this website , for our pharma students world wide .
We recomend you to sebscribe to this website and receive our articles directly by email when ever we update this website.

This website is most favorite Amongst proffesionals in almost all pharmaceutical companies in USA , Pharmaceutical companies in UK , Pharmaceutical companies in Japan , Canada , UAE , Pharmaceutical companies in India 

Following are some of articles which;will be useful for you in further understanding of aspects of sterile dosage form manufacturing

Pharmaceutical Validation

Cleen Room Classification

Pharmaceutical Industry

Pharmaceutical Manufacturing

Clinical Trials

Media fill

Regulatory Affairs in Pharmaceutical Industry

No comments:

How to successfuly activate and receive our articles on pharmaceuticals by E-Mail

Dear readers if you wish to receive our articles on pharmaceutical automatically into your email Inbox , as and when we write a new article over this website, then follow these 3 simple steps.

1.You should write your Email ID in the box provided and push the subscribe button

2.Enter the text code that we show you in a small new browser window after you enter your email ID.

3.Log in to your email and Check our verification email sent by "FEEDBURNER" , there you should verify the link we send you by email.

Enter your email address get our articles by email whenever this website is updated

Delivered by FeedBurner

Join the list of our readers from Universities ,Research and
Development centers Pharmaceutical Companies from all over the world.

Pharma Research Regulatory Guidelines Website
Texas Dallas, TX 75211 US
Email: Website:
Copyright © 2008-2020 all rights reserve