To 5ml of enzyme solution add 2ml of buffer of PH .2 and 1ml of fresh 1% sucrose solution. Mix well the contents of tube and allow to stand for 30 mins. Make the solution just alkaline with 1m Na2CO3 and perform Benedicts test [2ml of above solution + 2ml Benedicts reagent + 0.5gm of Na2CO3 and heat Red ppt. indicates +ve
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