Custom Search

Sunday, December 13, 2009

HOW TO ESTIMATE CONTENT OF DNA IN A GIVEN SAMPLE

How to determine the DNA content of given sample by diphenylamine reaction.

When DNA is treated with diphenylamine acidic conditions a blue compound is formed with sharp absorption maxima at 595 nm. This reaction is given by 2’- deoxy pentose in general and is not specific for DNA. In acid soln the straight chain form of deoxy ribose is converted to highly reactive &-hydroxyl laevulin aldehyde which reacts with biphenyl amine to give blue columned complex. In DNA only the deoxyribose of Purina nucleotide reacts so that the value obtained represents half of the total deoxyribose present.
Requirement: - 1) std. DNA soln (1 mg/ml)
2) Diphenylamine reagent

Procedure: - To 1 ml of sample and std. add 2 ml water add 5 ml of diphenylamine reagent. Heat the soln on a water bath for 10 mins. and cool measure absorbance at 595 nm against water blank.


========================================================================
This website is a guide for for pharmacy students , pharmaceutical professional working in pharmaceutical regulatory affairs , pharmaceutical manufacturing , quality assurance , pharmaceutical formulation development , research and development.
This week we are publishing a series of articles on biochemistry, over this website , for our pharma students world wide .
We recomend you to sebscribe to this website and receive our articles directly by email when ever we update this website.

Following are some of articles which;will be useful for you in further understanding of aspects of sterile dosage form manufacturing

Pharmaceutical Validation

Cleen Room Classification

Pharmaceutical Industry

Pharmaceutical Manufacturing

Clinical Trials

Media fill

Regulatory Affairs in Pharmaceutical Industry

No comments:

Posted by: Martin

How to sucessfuly activate and receive our articles on pharmaceuticals by E-Mail

Dear readers if you wish to receive our articles on pharmaceutical automatically into your email Inbox , as and when we write a new article over this website, then follow these 3 simple steps.


1.You should write your Email ID in the box provided and push the subscribe butten


2.Enter the text code that we show you in a small new browser window after you enter your email ID.

3.Log in to your email and Cheke our verification email sent by "FEEDBURNER" , there you should verify the link we send you by email.




Enter your email address get our articles by email whenever this website is updated





Delivered by FeedBurner



Join the list of our readers from Universities ,Research and Development centers Pharmaceutical Companies from all over the world.











Pharma Research Regulatory Guidelines Website
Texas Dallas, TX 75211 US
Email: editor@pharmacistspharmajournal.org Website: http://pharmacistspharmajournal.org/
Copyright © 2008-2015 all rights reserve www.pharmacistspharmajournal.org