Requirements: - Blood, 10% Sodium turnstile, 0.6 NH2SO4, std glucose solution [ 5mg / ml], alkaline cuso4 soln, phosphomolybdic acid.
Procedure: - Protein free filtrate of blood; to 1 ml of distilled water in a c.f. add 0.2 ml blood, wash ppt with 1 ml water and add to centrifuge. Add 0.2ml 10% Na- tang state and 0.2 ml 0.6N H2804 centrifuge or filter.
Standard curve: - Prepare a series of test tube with graded amount of glucose [0.05 to 0.2 mgs]. Add 2 ml of alkaline cu reagent. Heat tubes in a boiling water bath for 10 mins. Cool tubes to R.T.treat with 2 ml phosphomolybdic acid [from burette]. Make up volume to 10 ml with distilled water. Mix solution in each test tube and measure extinction at 420 nm against blank. Treat 1 ml sample in same manner and determine extinction determine glucose cone by extrapolation.
Express cone. of sugar in mg / 100 ml of glucose.
========================================================================
This website is a guide for for pharmacy students , pharmaceutical professional working in pharmaceutical regulatory affairs , pharmaceutical manufacturing , quality assurance , pharmaceutical formulation development , research and development.
This week we are publishing a series of articles on biochemistry, over this website , for our pharma students world wide .
We recomend you to sebscribe to this website and receive our articles directly by email when ever we update this website.
Following are some of articles which;will be useful for you in further understanding of aspects of sterile dosage form manufacturing
Pharmaceutical Validation
Cleen Room Classification
Pharmaceutical Industry
Pharmaceutical Manufacturing
Clinical Trials
Media fill
Regulatory Affairs in Pharmaceutical Industry
No comments:
Post a Comment