HOW TO ESTMATE OF LIPASE ACTIVITY
Lipase is a fat splitting enzyme. It hydrolyses fats into fatty acids and glycero
Trigycerides +Lipase enzyme------------------->Fatty Acids +Glycerin
For lipase optimum PH is around 7-7.2 and optimum 10 mp is 370c.The enzyme unit I.O amount of enzyme that liberates fatty acid which consumes 1 ml of 0.05 m alkali at 370c and optimum PH after 3 hrs. of incubation.
Requirements: - Enzyme source: 1) 2% pancreatic. 2) 5% germinated castor oil seed extract – Acetone: Alcohol 1:1, NaOH 0.05 N, substrate. (Groundnut oil oxidize)
Procedure: - To 10 ml of substrate add 10 ml of enzyme extract incubate at 37%c for 3 hrs. After incubation add 30 ml of acetone alcohol mixture to using in inactive the enzyme. Titrate fatty acid liberated using phenol ptyalin indicator. Carry out blank using inactivated blank extract.
Enzyme unit u = amount of enzyme required to produce fatly acid equivalent to 1 ml of 0.05 NaOH at end of 3 hrs.
Preparation of substrate: 100 ml oil + 400 ml H2o + 40 gm gum acacia.
========================================================================
This website is a guide for for pharmacy students , pharmaceutical professional working in pharmaceutical regulatory affairs , pharmaceutical manufacturing , quality assurance , pharmaceutical formulation development , research and development.
This week we are publishing a series of articles on biochemistry, over this website , for our pharma students world wide .
We recomend you to sebscribe to this website and receive our articles directly by email when ever we update this website.
Following are some of articles which;will be useful for you in further understanding of aspects of sterile dosage form manufacturing
Pharmaceutical Validation
Cleen Room Classification
Pharmaceutical Industry
Pharmaceutical Manufacturing
Clinical Trials
Media fill
Regulatory Affairs in Pharmaceutical Industry
Custom Search
Sunday, December 13, 2009
ESTMATION OF LIPASE ACTIVITY
Subscribe to:
Post Comments (Atom)
How to successfuly activate and receive our articles on pharmaceuticals by E-Mail
Dear readers if you wish to receive our articles on pharmaceutical automatically into your email Inbox , as and when we write a new article over this website, then follow these 3 simple steps.
1.You should write your Email ID in the box provided and push the subscribe button
2.Enter the text code that we show you in a small new browser window after you enter your email ID.
Development centers Pharmaceutical Companies from all over the world.
1.You should write your Email ID in the box provided and push the subscribe button
2.Enter the text code that we show you in a small new browser window after you enter your email ID.
3.Log in to your email and Check our verification email sent by "FEEDBURNER" , there you should verify the link we send you by email.
No comments:
Post a Comment