Guide for Pharmaceutical Manufacturing Industry Research & Development Medical Pharmacy Students FDA updates Guidelines
Custom Search

Friday, April 24, 2009

Phosphatase enzyme determine in Milk complete test for pasteurization of milk

Test for Phosphatase enzyme in Milk to determine complete pasteurization of milk
Phosphatase Test on Milk:-
Principle: - Phosphatase is an enzyme present in raw milk which is destroyed by adequate pasteurization, thus one can determine whether the milk has been properly pasteurized by testing for the absence of this enzyme. The milk which in its raw conditions when added to a substrate, disodium phenyl phosphate releases phenol which can be conveniently detected by colorimetric reaction with a suitable reagent.

Disodium phenyl phosphate + Phosphates ---> Phosphate +Phenol+Reagent

Procedure:-Mix 0.5ml of milk with 10ml of sodium veronaldisodium phenyl phosphate buffer solution and incubate at 370c for 10min. Add 4 drops of 2,6 dibromo quinine chlorimide solution and keep it for colour development, allow the colour to develop. After 5min. shake with 3 ml. of n-butyl alcohol. Blue colour in n-butyl alcohol indicates presence of phosphates and hence improperly pasteurized milk. A control test was performed on sample of pasteurized milk and the test sample was compared with it. A faint blue colour appeared in pasteurized milk which was used for the comparison test for pasteurization of milk
This website is a place where you will find complete information about
Pharmaceuticals cgmp quality systems quality assurance quality control fda approval standard operating procedures regulatory affairs it is very useful for pharmacists

No comments:

Like This Website on Facebook and Stay Connected Share This Article with your Friends

Latest Pharma Update

USFDA approves first drug Inmazeb to treat Ebola Virus Infection

US FDA approved first drug to treat Ebola Virus Infection (zaire ebolavirus) . So far there was no drug treatment available for Ebola virus ...

You May Also Like

If you are looking for latest Pharmaceutical Manufacturing Guidelines of USFDA, UKMHRA, TGA WHO GMP then this website is one of most popular source.
Subscribe to this website by providing your email

Enter your email address get latest Pharma Guideline and technology update by email whenever this website is updated