This website is written by experts in Pharmaceutical Industry Manufacturing Research and Development and Regulatory Affairs Since 2008 and in 2011 Awarded as one of top 10 websites across world in Pharmaceutical Manufacturing Regulatory
USFDA WHO GMP Guidelines Pharmaceutical Regulations Information about Pharma Quality Managment System Validations Process Validation Cleaning Validation Equipment Qualification Equipment Process Validation Computer System Validation Sterile Product Manufacturing Guidelines Media fill guideline Aseptic Techniques in Sterile dosage form Clean Room Clean Room Classification Guidelines on Area Qualification HVAC AHU HEPA Filter HEPA Filter integrity testing and leak test

Friday, April 24, 2009

IMVIC Test series of tests different E coli Aerobacter acrogens

IMVIC Test
These are the series of tests employed to different late E coli from Aerobacter acrogens.
I--- Indole test. M-- Methyl red test, v-- Voges proskaver, i--> euphony
And C---> growth in citrate medium.

These two species bear a close resembalance to each other in their morphological and culture characteristics, Hence it is necessary to resort to biochemical tests for their diffrentiation.
Indole Test:- In dole is a nitrogen containing compound formed from the degradation of the amine acid tryptophan only. Certain bacteria from it and can be detected chemically usually digested products of proteins like try tone are used in the medium since they contain considerable amount of tryptophan.

Tryotophan + H2O ---> Idol + Pyruvic acid

Inoculate try tone broth tubes with E coli incubate for 48 hrs. Add 0.5ml of covax’s reagent and shake, a red colour indicates +ve test Methyl red and vogues park Auer Reaction . Both the tests are considered together because, they can be performed together on the same medium and they one both of important in characterisation of gram –ve, non spore forming rods Instead of accumulating termentation products of glucose, some bacteria convert them to an informed ate subs, pyruvic acid to neutral products Co2. The formation of most easily detected neutral products is acetyl methyl carbinol or acitoin ( CH3 CO CH OH CH3 ). This test is also called as Vogues Pork Auer Reaction. Organism which so not converts acid and pdts to neutral product a final PH lower than those which do. By employing the indicator methyl red cyellow at PH ,Red at PH 4.4 ). Organisms own be distinguished, This is methyl red test. Inoculate two tubes of MRVP medium with E .coil and Aerobatic derogenes resp. Incubate for 48 hrs
====================================================================
This website is a place where you will find complete information about
Pharmaceuticals cgmp quality systems quality assurance quality control fda approval gmp guidelines standard operating procedures regulatory affairs it is very useful for pharmacists

No comments:

Pharma Guidelines Most Read

.

Readers Visit Number

This website is Authentic Source of Pharmaceutical Guidelines

This website is Authentic Source of Pharmaceutical Guidelines

Pharma Validations Quality Management System USFDA Guidelines WHO GMP Guidelines New Drug Update





This website consist of detail Information about Pharma Guidelines Pharma Regulatory Affairs USFDA Guidelines WHO Guidelines Pharmaceutical Quality Management System Pharma Validations Process Validation Cleaning Validation Equipment Qualification Equipment Process Validation Computer System Validation Sterile Product Manufacturing Guidelines Media fill guideline Aseptic Techniques in Sterile dosage form Clean Room Clean Room Classification Guidelines on Area Qualification HVAC AHU HEPA Filter HEPA Filter integrity testing and leak test