This website is written by experts in Pharmaceutical Industry Manufacturing Research and Development and Regulatory Affairs Since 2008 and in 2011 Awarded as one of top 10 websites across world in Pharmaceutical Manufacturing Regulatory
USFDA WHO GMP Guidelines Pharmaceutical Regulations Information about Pharma Quality Managment System Validations Process Validation Cleaning Validation Equipment Qualification Equipment Process Validation Computer System Validation Sterile Product Manufacturing Guidelines Media fill guideline Aseptic Techniques in Sterile dosage form Clean Room Clean Room Classification Guidelines on Area Qualification HVAC AHU HEPA Filter HEPA Filter integrity testing and leak test

Friday, April 24, 2009

Phenol coefficient Rideal walker coefficient calculation disinfectant antimicrobial agent

Determination of phenol coefficient (Rideal walker coefficient) of a given disinfectant or antimicrobial agent.

Phenol coefficient may be defined as the killing power of germicide or an antimicrobial agent towards a test organism compared to that of phenol under identical conditions. It is the ratio of the higher dilation of disinfectant killing the organism in 10min. but not in 5min, to the of the highest dilution of phenol showing the same result.
Materials: - 24 hrs. Broth culture of S.aureus
Sterile test tube (10)
Platinum 100p
Nutrient broth (10ml per tube)
Distilled water.
Sterile diln flask.
Sterile 5 and 10ml graduated pipettes.
Various dilution of phenol.
Various dilution of disinfectant under test.
Procedure:-
Place 5ml of various dilutions of phenol and disinfectant under test into sterile test tube Label them from 1 to 10, place 0.5ml of broth culture of test organism into tubes no1, shake to distribute the organism and at 30sec. internal thereafter add 0.5ml of culture of test organism in to tubes no1, shake to distribute the organism and at 30 sec. interval thereafter add 0.5ml of culture to the remaining tubes containing various dilutions. When tube no.10 is inoculated 41/2 min will be passed 30 second later proceeding as follows.
Place one standard loopful from tube no1 to the tube of broth and at 30sec. internal thereafter transfer a loopful of each successive disinfecting mix. This will give 3 series of inoculation to a fresh tube of broth until 3 transfers have been made from each disinfecting mix. This will give 3 series of inoculation in to broth after 5,10 and 15 min exposure of test organisms, through various concentrations. of disinfectant. In cubit broth tubes at 370c for 48hrs. Record in a tubular from presence or absence of growth.


Various dilutions of phenol are done on follows shown in picture to determine Phenol coefficient














Interpretation of results for phenol coefficient


calculation of phenol coefficient



=====================================================================
This website is a place where you will find complete information about FDA guidelines
Pharmaceuticals cgmp quality systems quality assurance quality control  gmp guidelines standard operating procedures regulatory affairs , clinical trials it is very useful for pharmacists it is one of the most referred website in pharmaceutical companies all over the world

No comments:

Pharma Guidelines Most Read

.

Readers Visit Number

This website is Authentic Source of Pharmaceutical Guidelines

This website is Authentic Source of Pharmaceutical Guidelines

Pharma Validations Quality Management System USFDA Guidelines WHO GMP Guidelines New Drug Update





This website consist of detail Information about Pharma Guidelines Pharma Regulatory Affairs USFDA Guidelines WHO Guidelines Pharmaceutical Quality Management System Pharma Validations Process Validation Cleaning Validation Equipment Qualification Equipment Process Validation Computer System Validation Sterile Product Manufacturing Guidelines Media fill guideline Aseptic Techniques in Sterile dosage form Clean Room Clean Room Classification Guidelines on Area Qualification HVAC AHU HEPA Filter HEPA Filter integrity testing and leak test