Custom Search

Sunday, December 13, 2009

How to estimate blood Glucose by folin –wu method

Estimation of Blood Glucose by Folin – wu method Glucose quantitatively reduces ul baric ions to cuprous ions in a boiling alkaline solution. It gets oxidized to glycolic acid, consequently reduce phosphomolybdic acid [Mo +6 Mo +4] are themselves oxidized back to cubic ions. The reduced form phosphonolybdic acid imparts a blue co lour which is read calorimetrically.

Boiling sugar is mainly glucose normal blood sugar levels in adult human subject in the post absorptive absorptive state (overnight fast) varies from 60 – 110 mg / 100 ml. No difference in blood sugar is found with r.t. sex. A blood sugar level is found to be raised by 10 – 20 mg / 100 mg in state of anger fear anxiety and pain. After carbohydrate digestion, blood sugar level may rise to 130 – 140 mg / 100 ml and after 1 – 3 hrs. Come back to normal. This fall is due to consumption thro glycolysis. TCA cycle, HMP pathway, glycogen sis and biogenen’s of lipids and proteins. As blood proteins interfere c estimation they must be ppted before proceeding for determination. Blood sugar must be level are normally under normal control.


Requirements: - Blood, 10% Sodium turnstile, 0.6 NH2SO4, std glucose solution [ 5mg / ml], alkaline cuso4 soln, phosphomolybdic acid.

Procedure: - Protein free filtrate of blood; to 1 ml of distilled water in a c.f. add 0.2 ml blood, wash ppt with 1 ml water and add to centrifuge. Add 0.2ml 10% Na- tang state and 0.2 ml 0.6N H2804 centrifuge or filter.

Standard curve: - Prepare a series of test tube with graded amount of glucose [0.05 to 0.2 mgs]. Add 2 ml of alkaline cu reagent. Heat tubes in a boiling water bath for 10 mins. Cool tubes to R.T.treat with 2 ml phosphomolybdic acid [from burette]. Make up volume to 10 ml with distilled water. Mix solution in each test tube and measure extinction at 420 nm against blank. Treat 1 ml sample in same manner and determine extinction determine glucose cone by extrapolation.
Express cone. of sugar in mg / 100 ml of glucose.


========================================================================
This website is a guide for for pharmacy students , pharmaceutical professional working in pharmaceutical regulatory affairs , pharmaceutical manufacturing , quality assurance , pharmaceutical formulation development , research and development.
This week we are publishing a series of articles on biochemistry, over this website , for our pharma students world wide .
We recomend you to sebscribe to this website and receive our articles directly by email when ever we update this website.

Following are some of articles which;will be useful for you in further understanding of aspects of sterile dosage form manufacturing

Pharmaceutical Validation

Cleen Room Classification

Pharmaceutical Industry

Pharmaceutical Manufacturing

Clinical Trials

Media fill

Regulatory Affairs in Pharmaceutical Industry

No comments:

Posted by: Martin

How to sucessfuly activate and receive our articles on pharmaceuticals by E-Mail

Dear readers if you wish to receive our articles on pharmaceutical automatically into your email Inbox , as and when we write a new article over this website, then follow these 3 simple steps.


1.You should write your Email ID in the box provided and push the subscribe butten


2.Enter the text code that we show you in a small new browser window after you enter your email ID.

3.Log in to your email and Cheke our verification email sent by "FEEDBURNER" , there you should verify the link we send you by email.




Enter your email address get our articles by email whenever this website is updated





Delivered by FeedBurner



Join the list of our readers from Universities ,Research and Development centers Pharmaceutical Companies from all over the world.











Pharma Research Regulatory Guidelines Website
Texas Dallas, TX 75211 US
Email: editor@pharmacistspharmajournal.org Website: http://pharmacistspharmajournal.org/
Copyright © 2008-2015 all rights reserve www.pharmacistspharmajournal.org