Custom Search

Sunday, November 22, 2009

How to estimate content of RNA in a given sample by orcinol method

Estimation of RNA by Orcinol Method
To estimate RNA in a given sample by orcinol method.

When a solution of RNA is treated with orcinol. Reagent, it gets hydrolyzed to component base, phosphate and rebase reduce. Further heating with orcinol produces bluish green complex in presence of Fe+3.

Requirement: - Std. soln of RNA [0.05 mg/ml] sample solution of RNA, fresh orcinol reagent.

Procedure: - Mix 2 ml of sample and standard solution of RNA with 3 ml of orcinol reagent. Heat on boiling water bath for 30 mins. Cool and determine extinction at 665 nm. Against oreinol blank.







Calculation: - Conc. of sample = (Conc. Of standard / Absorbance of std.)x Absorbance of sample
                              

= 0.05 /0.249* 0.448


= 0.8945 mg / ml.

The conc. of RNA in given sample was found to be 0.8945 mg/ml.

===================================================================

This website is a guide for pharmaceutical manufacturing and for pharmacy students and pharmaceutical regulatory affairs proffesionals and for every one associated with pharmaceutical industry , we recommend you to subscribe to this website by entering your email ID in the box provided so that you will receive our articles as and when our website is udated.

This website is most favorite Amongst proffesionals in almost all pharmaceutical companies in USA , Pharmaceutical companies in UK , Pharmaceutical companies in Japan , Canada , UAE , Pharmaceutical companies in India 

Following are some of articles which;will be useful for you in further understanding of aspects of sterile dosage form manufacturing

Pharmaceutical Validation

Cleen Room Classification

Pharmaceutical Industry

Pharmaceutical Manufacturing

Clinical Trials

Media fill

Regulatory Affairs in Pharmaceutical Industry

1 comment:

QUALITY SERVICES said...

Nice post, I bookmark your blog because I found very good information on your blog, Thanks for sharing- more information
http://goo.gl/tGqfRs

Posted by: Martin

How to sucessfuly activate and receive our articles on pharmaceuticals by E-Mail

Dear readers if you wish to receive our articles on pharmaceutical automatically into your email Inbox , as and when we write a new article over this website, then follow these 3 simple steps.


1.You should write your Email ID in the box provided and push the subscribe butten


2.Enter the text code that we show you in a small new browser window after you enter your email ID.

3.Log in to your email and Cheke our verification email sent by "FEEDBURNER" , there you should verify the link we send you by email.




Enter your email address get our articles by email whenever this website is updated





Delivered by FeedBurner



Join the list of our readers from Universities ,Research and Development centers Pharmaceutical Companies from all over the world.











Pharma Research Regulatory Guidelines Website
Texas Dallas, TX 75211 US
Email: editor@pharmacistspharmajournal.org Website: http://pharmacistspharmajournal.org/
Copyright © 2008-2015 all rights reserve www.pharmacistspharmajournal.org