1] Cylinder plate.
2] Cup plate and
3] Paper disc method.
The term Cylinder plate is applied to the method in which small cylinders are placed on a surface of the agar plates and term.
How to do Preparation: Prepare the plates by adding exactly 15ml of nutrient agar to each sterile, Petri dish aseptically. Allow it to set, Incubate the plate for 24 hrs as a test for sterility. Inoculate sterile nutrient agar which has been melted and cooled to 500c with a 24hrs broth (culture grown in liquid medium). Culture or s.aurem in proportion of (1:100)
Mix well and pour in the Petri dish.Place petridishes on horizontal surface until agar stets.
Use the plates for assay of penicillin by any of the following 3 methods.
1] Cylinder plate method: - Place (4) sterile cylinder in each Petri dish by dropping them at a height of 1/8th of inch in agar surface. Place each cylinder equidistance from center. By sterile pipettes fill each cylinder with standard solution penicillin containing 0.5, 1, 1.5, 2, and 2.5. I units/ml respectively. Fill other cylinder with sample to be tested. Place the plates carefully and incubate of 370c for 18hrs. Measure diameter of zone of inhibition. Take average measurements of stds and the test sample.
2] Cup plate method: - In the seeded agar plates cut out cylindrical cups by means of cork borer (diameter about 3.5mm). Remove dishes by means of small pointed spheres. Place identical volume (0.2ml) of various test solution and standers in the cups. Incubate at 370c for 18hrs.compare diameters of zone of inhibition as before.
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